Differences in tagging Gram-negative and positive bacteria?

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Discussion Overview

The discussion centers around the differences in tagging Gram-negative and Gram-positive bacteria using fluorescent proteins such as GFP and mCherry. Participants explore whether the principles of tagging are similar for both types of bacteria and the implications of plasmid and promoter compatibility.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested

Main Points Raised

  • One participant questions whether the principles of tagging Gram-negative bacteria with fluorescent proteins are similar to those for Gram-positive bacteria.
  • Another participant suggests that the fluorescent protein itself should not matter, referencing an article to support this claim.
  • Concerns are raised regarding plasmid compatibility and promoter recognition, indicating that these factors may differ between Gram-negative and Gram-positive bacteria.
  • A later reply acknowledges the assistance provided, indicating ongoing engagement in the discussion.

Areas of Agreement / Disagreement

Participants do not reach a consensus on whether the principles of tagging are the same for Gram-negative and Gram-positive bacteria, and multiple views regarding the importance of plasmid and promoter compatibility remain present.

Contextual Notes

The discussion does not resolve the specific differences in tagging methodologies or the implications of plasmid and promoter interactions for different bacterial types.

Skwrl
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Hi there,

Recently I've been reading about tagging bacteria with GFP, mCherry and other fluorescent proteins. The article I'm reading now (Lagendijk et at., 2010, Genetic tools for tagging Gram negative bacteria with mCherry...) has information on tagging Gram-negative bacteria, but it doesn't say anything about Gram-positive ones. Is the principle similar or is tagging with marker genes different when the bacteria are Gram-positive?

Thank you in advance!
 
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As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.
 
iansmith said:
As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.
Hey Ian! Good to see you back!
 
Thanks for the help!
 

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