Differences in tagging Gram-negative and positive bacteria?

[Skwrl]

Hi there,

Recently I've been reading about tagging bacteria with GFP, mCherry and other fluorescent proteins. The article I'm reading now (Lagendijk et at., 2010, Genetic tools for tagging Gram negative bacteria with mCherry...) has information on tagging Gram-negative bacteria, but it doesn't say anything about Gram-positive ones. Is the principle similar or is tagging with marker genes different when the bacteria are Gram-positive?

Thank you in advance!

 

[iansmith]

As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.

 

[Evo]

As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.

Hey Ian! Good to see you back!

 

[Skwrl]

Thanks for the help!