Differences in tagging Gram-negative and positive bacteria?

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SUMMARY

The discussion focuses on the differences in tagging Gram-negative and Gram-positive bacteria using fluorescent proteins such as GFP and mCherry. While the fluorescent proteins themselves do not affect the tagging process, the choice of plasmids and promoters is critical due to compatibility issues and varying activity levels. The article by Lagendijk et al. (2010) specifically addresses Gram-negative bacteria, leaving a gap in information for Gram-positive tagging methods. Researchers must consider these factors when designing experiments for bacterial tagging.

PREREQUISITES
  • Understanding of fluorescent protein tagging techniques
  • Knowledge of plasmid compatibility and promoter activity
  • Familiarity with Gram-negative and Gram-positive bacterial characteristics
  • Basic principles of molecular biology and genetic engineering
NEXT STEPS
  • Research the differences in plasmid compatibility for Gram-positive bacteria
  • Explore various promoter systems suitable for Gram-positive bacteria
  • Investigate alternative fluorescent proteins for bacterial tagging
  • Review the article by Lagendijk et al. (2010) for insights on Gram-negative tagging
USEFUL FOR

Microbiologists, genetic engineers, and researchers involved in bacterial tagging and genetic modification will benefit from this discussion.

Skwrl
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Hi there,

Recently I've been reading about tagging bacteria with GFP, mCherry and other fluorescent proteins. The article I'm reading now (Lagendijk et at., 2010, Genetic tools for tagging Gram negative bacteria with mCherry...) has information on tagging Gram-negative bacteria, but it doesn't say anything about Gram-positive ones. Is the principle similar or is tagging with marker genes different when the bacteria are Gram-positive?

Thank you in advance!
 
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As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.
 
iansmith said:
As far as the fluorescent protein, it should not matter. Look at this article for example:
http://www.ncbi.nlm.nih.gov/pubmed/19264102

However, plasmids and promoter will matter. It has to do with plasmid compatibility and not every promoter will recognized or have the same activity.
Hey Ian! Good to see you back!
 
Thanks for the help!
 

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