Differentiation of cells in an embryo

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Discussion Overview

The discussion centers on the differentiation of cells in an embryo, particularly whether differentiation can begin before implantation in the uterus, and the mechanisms that regulate this process. It explores various stages of embryonic development, including the implications for phenomena such as conjoined twins.

Discussion Character

  • Exploratory
  • Debate/contested
  • Technical explanation

Main Points Raised

  • Some participants suggest that embryo cells typically start differentiating at implantation, while others argue that differentiation begins earlier, potentially with the first division.
  • It is noted that blastocysts are already on a path of differentiation, with distinct roles for the trophoblast and inner cell mass.
  • A participant mentions that embryonic cells may have mechanisms to turn genes on or off to determine their positional development in the body.
  • One participant references research indicating that embryonic cells may not all develop into different cell types independently, citing an experiment involving heart cell development.
  • Another participant states that in vertebrates, differentiation does not begin until after the 8-cell stage, raising questions about the mechanisms preventing differentiation before this stage.
  • A later reply proposes that a transcriptionally repressive state may develop during the 2-cell stage, which could inhibit differentiation until specific signals are received from surrounding cells.
  • Concerns are raised about the development of conjoined twins, questioning how they can form if differentiation does not occur before the 8-cell stage.
  • One participant clarifies that conjoined twins result from incomplete splitting of the embryo around the 8-cell stage, suggesting a specific time window for this occurrence.

Areas of Agreement / Disagreement

Participants express differing views on when differentiation begins in embryos, with some asserting it starts at implantation and others suggesting it occurs earlier. The discussion remains unresolved regarding the exact mechanisms that prevent differentiation before the 8-cell stage and the implications for conjoined twins.

Contextual Notes

There are limitations in the discussion regarding the specific timing of differentiation and the exact biological mechanisms involved, which remain speculative and not fully understood.

sontag
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Can embryo cells start to differentiate before the embryo
is implanted in the uterus? The differentiation usually starts
at the time of implantation.
 
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Blastocysts are implanted, these are already on their path of differentiation (trophoblast/inner cell mass).
 
Embryoes likely begin differentiating with the first division. In order for areas of the body to develop in the appropriate position on the body the cells need to know their position in the body. This would most likely be accomplished by cells initially having a mechanism to turn groups of genes on or off so that they develop for the appropriate portion of the body.

Some research indicates that embryonic cells cannot each develop into all different cells. For example, an experiment to develop heart cells had to rely on watching for some of the embryonic cells in a dish to develop into cells that acted like heart cells.

http://www.washingtonpost.com/wp-dyn/articles/A52471-2004Sep26.html
 
In vertebrates, the cells do not begin differentiating until after the 8-cell stage. Up until this point, the embryo could split in half and form twins.
 
What stops cells differentiating in vertebrates before the 8 cell stage?
 
VERY good question :) I'm not sure if there is a definative answer to that. Schultz et. al. (1999) proposed that a transcriptionally repressive state develops during the 2-cell stage (that transforms highly differentiated oocytes into totipotent blastomeres), where an increased histone deacetylation represses transcription.

The whole transcription machinery needs to be started again, which is sensitive to signals from the cells it is surrounded with. For me it is speculation what exactly happens at these very early stages, probably it is competition where the cells are competing against chemokines excreted by the others, when one cell takes the overhand it starts producing negative regulatory proteins that inhibit the other cells to differentiate in the same way. This then sets off a cascade that triggers the other cells to undertake other developmental routes.

You can read up on developmental biology to understand exactly how cells communicate are able to create complex architectures.
 
nipwoni said:
In vertebrates, the cells do not begin differentiating until after the 8-cell stage. Up until this point, the embryo could split in half and form twins.

If no differentiation occurs, how do you explain conjoined twins? they develop as mirror images connected at different locations, typically the torso or head, and occasionally share organs. Something in the programming causes both to develop heads at the same end and feet at the same end, unless you know of cases in which one develops upside down compared to the other.
 
That is the whole point: you get conjoined twins if the egg incompletely splits around the 8-cell stage (not sure exactly what the time window is).
 

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