Enzyme kinetics plotting a slope replot

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Discussion Overview

The discussion revolves around diagnosing enzyme inhibition types, specifically focusing on competitive inhibition and the process of creating a slope replot to determine the inhibition constant (KI). Participants explore the methodology of slope replots and the graphical representation of their data.

Discussion Character

  • Homework-related
  • Technical explanation
  • Conceptual clarification

Main Points Raised

  • One participant questions whether the slope for the slope replot can be derived from the linear regression line generated by Excel, expressing confusion over the resulting hyperbolic shape of their graph.
  • Another participant suggests that providing more details about the plots and the specific data being used could facilitate better assistance.
  • There is a mention of the importance of dialogue and feedback in the learning process, highlighting the frustration of not receiving responses after asking questions.
  • A later reply references the possibility that the method being discussed might relate to the Dixon plot, indicating an exploration of different approaches to the problem.

Areas of Agreement / Disagreement

Participants express varying levels of understanding regarding the slope replot process, and there is no consensus on the best approach or interpretation of the results. The discussion remains unresolved with multiple viewpoints presented.

Contextual Notes

There are limitations in the discussion regarding the clarity of the plots and the specific data being analyzed, which may affect the understanding of the slope replot process. Additionally, assumptions about the participants' prior knowledge and the context of the assignment are not fully articulated.

Who May Find This Useful

This discussion may be useful for students working on enzyme kinetics, particularly those dealing with competitive inhibition and slope replots in their assignments.

skyblu280
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hi,

My instructor has given us an assignment where we have to diagnose the type of enzyme inhibition it is. I diagnosed it as a competitive inhibitor and am trying to do a slope replot to find my value for KI.

For the slope replot, you just find the slope of the each line? is it the same as having excel find the linear regression line and taking that slope to make a slope replot? Because that's why I did but my graph is turning kind of hyperbolic
 
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skyblu280 said:
hi,

My instructor has given us an assignment where we have to diagnose the type of enzyme inhibition it is. I diagnosed it as a competitive inhibitor and am trying to do a slope replot to find my value for KI.

For the slope replot, you just find the slope of the each line? is it the same as having excel find the linear regression line and taking that slope to make a slope replot? Because that's why I did but my graph is turning kind of hyperbolic

We are of course incredibly brilliant but even we have some limitations.

E.g. we are fairly telepathic but not totally. E.g. to be on the safe side it would be better to tell us just what you are plotting and replotting.

We might also get ideas better if you posted pics of your plots.

Another of our defects is some of us are touchy, ratty or peevish. If we answer one of your questions and hear no response ever :wink: we might wonder whether to try and help out the next time. :-p :smile:
 
Hi,

Sorry for not thanking you earlier lol. But I think got the slope replot thing down.
 
skyblu280 said:
Hi,

Sorry for not thanking you earlier lol. But I think got the slope replot thing down.

It's not about thanks, it's about thinking through an answer in a dialogue and then being left in the air. Like if you gave your results, plots and conclusions to your Prof. and then never head a thing.

Anyway glad you have sorted it.

Maybe this was the thing called the Dixon plot.
 

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