Freezing temperature of vaccines

[hagopbul]

TL;DR

Asking about the nano droplets and freezing temperature of liquid that holding it

Hello all:

I have this question , as we all watch the vaccine of Covid-19 reach the market I had this question if the storage temperature of this vaccine is between -20 c to -70c

Why the nano droplets in this liquid do not create ice the liquid is not freezing under that conditions although it is full of nano droplets contains mRNA and spike proteins

What did they use ?

Best
H.B.

 

[Ygggdrasil]

The issue is not the freezing point of the solution, but the stability of the RNA molecules. mRNA molecules are designed to be short lived and are therefore very susceptible to degradation (the sugar-phosphate backbone is capable of binding to and activating common metal ions, like Mg⁺⁺, to catalyze the degradation of the RNA molecule). It is very common in laboratories to store RNA at -70°C (e.g. see the "How to Store RNA" section of the website below):
https://www.thermofisher.com/us/en/...nzymes/general-articles/working-with-rna.html

 

[.Scott]

From https://www.fda.gov/media/144637/download

Storage Prior to Use
As Displayed on the Vial Labels and Cartons:
The Moderna COVID-19 Vaccine multiple-dose vials are stored frozen between -25º to -15ºC (-13º to 5ºF). Store in the original carton to protect from light.

Additional Storage Information Not Displayed on the Vial Labels and Cartons:
Do not store on dry ice or below -40ºC (-40ºF). Vials can be stored refrigerated between 2° to 8°C (36° to 46°F) for up to 30 days prior to first use.
Unpunctured vials may be stored between 8° to 25°C (46° to 77°F) for up to 12 hours
.Do not refreeze once thawed.

It seems that they are fully frozen.

 

[hagopbul]

are the freezing , then melting of the liquid is effecting the nano droplet that enclose the rna , wouldn't that melting do or better say damage the mRNA

do the liquid that hold the droplets have the same freezing temperature of the droplets or they do not freeze ?