- #1
David23454
- 31
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Homework Statement
Rewrite following sequence as double stranded molecule, with an inversion of the sequence between nucleotide 4 and 12.
5'-TCTCGCATGGTAGGT-3'
Homework Equations
none.
The Attempt at a Solution
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I've read in the textbook that you take the section and rotate it 180 degrees, then reinsert it. However I think that I'm not understanding this properly because when I do this, I get a 5' end that needs to be rejoined with another 5' end, and likewise with the 3' end:
Original: 5'-TCTCGCATGGTAGGT-3'
Taking out sequence which needs to be inverted: 5'-GCATGGT-3'
Invert: 3'-TGGTACG-5'
Inverted sequence:5'-TCTC-3' (3'-TGGTACG-5') 5'-AGGT-3' ...so I don't see how I can rejoin the inverted segment.
I feel like I'm missing something obvious, if someone could help me out with this it would be great.