SUMMARY
To prepare 100ml of a 0.1M sodium phosphate buffer at pH 7.6, utilize the NaH2PO4/Na2HPO4 buffer system, which is effective within the pH range of 6 to 8. The Henderson-Hasselbalch equation is essential for determining the correct proportions of the acids and salts involved. The pKa values of the components must be referenced to achieve the desired pH. Analytical chemistry handbooks provide valuable mathematical guidance for this preparation.
PREREQUISITES
- Understanding of buffer systems, specifically NaH2PO4 and Na2HPO4.
- Familiarity with the Henderson-Hasselbalch equation.
- Knowledge of pKa values relevant to phosphate buffers.
- Basic laboratory skills for preparing chemical solutions.
NEXT STEPS
- Research the Henderson-Hasselbalch equation in detail.
- Study the pKa values of phosphoric acid and its conjugate bases.
- Learn about the preparation and use of sodium phosphate buffers in laboratory settings.
- Explore analytical chemistry handbooks for comprehensive buffer preparation techniques.
USEFUL FOR
Chemists, laboratory technicians, and students in biochemistry or analytical chemistry who require knowledge on buffer preparation and pH management.