How to Calculate Transformation Efficiency?

Click For Summary

Discussion Overview

The discussion revolves around calculating transformation efficiency in a biological context, specifically focusing on the formula and the challenges associated with determining the amount of DNA plated. Participants explore theoretical aspects and practical issues related to experimental outcomes.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested, Experimental/applied

Main Points Raised

  • One participant inquires about the method to calculate transformation efficiency.
  • Another participant suggests a resource that may provide relevant information.
  • A participant shares a formula for transformation efficiency as colonies on plate divided by the amount of DNA plated in micrograms, expressing uncertainty about the accuracy of their calculations.
  • This same participant notes that a website indicates transformation efficiency should yield a large number, around 10^9, which raises doubts about their own results.
  • Another participant hints at potential issues with their experiment that may affect the results.

Areas of Agreement / Disagreement

Participants do not reach a consensus on the calculation method or the expected outcomes, indicating that multiple views and uncertainties remain in the discussion.

Contextual Notes

There are unresolved questions regarding the determination of the amount of DNA plated and the implications of experimental success on the calculated efficiency.

higherme
Messages
126
Reaction score
0
does anyone know how to calculate the Transformation efficiency?
 
Physics news on Phys.org
actually I kind of have the formula of the transformation efficiency

colonies on plate/ amount of DNA plated in (ug)

I just don't know how to find the amounf of DNA plated... well i kind of have an answer
but it's really small... but this website saids that the the transformation efficiency should be a big number... something like 10^9 that they got from their example...

so, i don't know if i am doing it right...
 
or maybe it's because my experient didn't work out that great
 

Similar threads

Chemistry What does this equation for an electrochemical cell mean?
  • · Replies 4 ·
Replies
4
Views
2K
Sizing an Isolation Transformer for a DC Power Supply
  • · Replies 8 ·
Replies
8
Views
3K
Chemistry How to find the pH of a galvanic cell (MIT OCW problem set)
  • · Replies 2 ·
Replies
2
Views
4K
MCNPX Transformation
  • · Replies 14 ·
Replies
14
Views
4K
Transformer frequency contradiction on induction heating
  • · Replies 3 ·
Replies
3
Views
2K
Enthelpy of Reaction and Efficiency of Galvanic Cells
  • · Replies 2 ·
Replies
2
Views
3K
Chemistry Work done by heat engine that uses exhaust from heat pump
  • · Replies 9 ·
Replies
9
Views
3K
Cloning & Transforming Bacterial Vector for Protein Production
  • · Replies 3 ·
Replies
3
Views
2K
Chemistry How Does Electrolyte Type Influence Voltage in Galvanic Cells?
  • · Replies 6 ·
Replies
6
Views
4K
Graduate Has Hilbert transform ever been used in Quantum Theory?
  • · Replies 3 ·
Replies
3
Views
2K