How to Calculate Transformation Efficiency?

[higherme]

does anyone know how to calculate the Transformation efficiency?

 

[Gokul43201]

Not covered in your text?

Try this: http://www.sciencebuddies.org/mentoring/project_ideas/BioChem_p013.shtml

 

[higherme]

actually I kind of have the formula of the transformation efficiency

colonies on plate/ amount of DNA plated in (ug)

I just don't know how to find the amounf of DNA plated... well i kind of have an answer
but it's really small... but this website saids that the the transformation efficiency should be a big number... something like 10^9 that they got from their example...

so, i don't know if i am doing it right...

 

[higherme]

or maybe it's because my experient didn't work out that great