HPLC column separating high polar compounds

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SUMMARY

The discussion focuses on the use of HPLC columns for separating high polar compounds, specifically recommending reverse phase columns such as C8 and C18. A C8 column is effective for separating nucleosides, while a C18 column is preferred for oligonucleotides. These choices are based on the polarity of the compounds being analyzed, ensuring optimal separation and analysis.

PREREQUISITES
  • Understanding of HPLC (High-Performance Liquid Chromatography) principles
  • Knowledge of reverse phase chromatography techniques
  • Familiarity with compound polarity and its impact on separation
  • Experience with specific HPLC column types, particularly C8 and C18
NEXT STEPS
  • Research the differences between C8 and C18 HPLC columns
  • Learn about optimizing HPLC methods for high polar compounds
  • Explore the impact of solvent choice on HPLC separation
  • Investigate advanced techniques for analyzing nucleosides and oligonucleotides
USEFUL FOR

Chemists, analytical scientists, and laboratory technicians involved in the separation and analysis of high polar compounds using HPLC techniques.

Tararosi
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TL;DR
Columns With The Function Of Separation For High Polar Compounds
Which HPLC column can separate high polar compounds?
 
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Typically one would use a reverse phase column, either C8 or C18 depending on the polarity (e.g. I use a C8 column to separate nucleosides and a C18 column to separate oligonucleotides).
 
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Ygggdrasil said:
Typically one would use a reverse phase column, either C8 or C18 depending on the polarity (e.g. I use a C8 column to separate nucleosides and a C18 column to separate oligonucleotides).
Really helpful. thanks!:)
 

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