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- Columns With The Function Of Separation For High Polar Compounds
Which HPLC column can separate high polar compounds?
The discussion focuses on the use of HPLC columns for separating high polar compounds, specifically recommending reverse phase columns such as C8 and C18. A C8 column is effective for separating nucleosides, while a C18 column is preferred for oligonucleotides. These choices are based on the polarity of the compounds being analyzed, ensuring optimal separation and analysis.
PREREQUISITESChemists, analytical scientists, and laboratory technicians involved in the separation and analysis of high polar compounds using HPLC techniques.
Really helpful. thanks!:)Ygggdrasil said:Typically one would use a reverse phase column, either C8 or C18 depending on the polarity (e.g. I use a C8 column to separate nucleosides and a C18 column to separate oligonucleotides).