HPLC column separating high polar compounds

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For separating high polar compounds using HPLC, reverse phase columns are recommended, specifically C8 or C18 columns. The choice between C8 and C18 depends on the polarity of the compounds being analyzed. C8 columns are effective for nucleosides, while C18 columns are suitable for oligonucleotides.
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Columns With The Function Of Separation For High Polar Compounds
Which HPLC column can separate high polar compounds?
 
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Typically one would use a reverse phase column, either C8 or C18 depending on the polarity (e.g. I use a C8 column to separate nucleosides and a C18 column to separate oligonucleotides).
 
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Ygggdrasil said:
Typically one would use a reverse phase column, either C8 or C18 depending on the polarity (e.g. I use a C8 column to separate nucleosides and a C18 column to separate oligonucleotides).
Really helpful. thanks!:)
 

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