In mesh analysis, the current in the middle of two loops should be consistently labeled to avoid confusion. When determining the relationship between currents, such as i1 and i2, it is essential to maintain a single label for each current throughout the equations. The subtraction of currents depends on the assumed direction; if the result is negative, it indicates that the initial assumption about the larger current was incorrect. Consistency in labeling and assumptions is crucial for accurate calculations. Understanding these principles will aid in effectively solving for middle loop currents.