Yes. The bus will start moving. Let's take an example. We have your 25 000 kg bus, and a 10 kg bike in space.
Now we exert a force of 2 N on both bodies. If we assume that we can neglect all opposing forces, (i.e. there is no friction, and gravitational forces are minimal) we can easilly calculate the acceleration the bus will get, and the acceleration the bike will get from
F=ma --> a=F/m
We see that the bus will get a smaller acceleration because it has a larger mass. In other words, when we stop applying our 2 N force on the bodies, the bike will have a larger velocity. That's what inertia is about. The heavier bus will not change it's state of motion as much as the bike. It's about comparison. Light object will get larger velocities than heavier objects when an equal force is applied on both bodies.
So back to your question, the bus will start moving with the lightest touch, but the bike will start moving a lot faster when the same touch is applied on it.
Neglecting gravity and other opposing forces