PH effects on membrane permeability

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SUMMARY

The discussion centers on the effects of pH on membrane permeability, specifically using beets and varying concentrations of HCl and NaOH. A notable observation was a strong color intensity in a test tube with 0.001 M NaOH, indicating significant membrane disruption. Participants emphasized the importance of understanding the role of pigments in beets and the protective membranes that prevent leakage. Formulating a hypothesis based on experimental observations is crucial for deeper analysis.

PREREQUISITES
  • Basic understanding of membrane biology
  • Knowledge of pH and its effects on cellular structures
  • Familiarity with spectrophotometry for measuring color intensity
  • Experience with laboratory techniques involving acids and bases
NEXT STEPS
  • Research the role of beet pigments in cellular membranes
  • Learn about the effects of different pH levels on membrane permeability
  • Investigate the use of spectrophotometry in measuring color intensity
  • Explore hypotheses related to membrane disruption by acids and bases
USEFUL FOR

Students in biology or chemistry, laboratory technicians, and educators looking to understand the relationship between pH and membrane permeability in plant cells.

scarling
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I had this lab done where we took beets and tested the effects of pH on membrane permeability.

We used different concentrations of HCl and NaOH. Why did I get such a strong colour intensity with a test tube that had 0.001 M NaOH? And how do I know this experiment affected the protein membrane? These are probably silly questions to ask.
 
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Welcome to the PF forums! if you haven't already looked around, you will find a lot of interesting and informative discussions here.

If you have homework questions like this one about a lab you are working on, you should explain what you have learned so far about your experiment. Can you think of a reason why a certain concentration of NaOH or HCl would produce a strong colour? Perhaps you can do some reading about pigments in beets and find out what kind of membranes protect them from leaking out of the cells. Then you could formulate an hypothesis to explain your results. The main thing is to show us your thoughts, then we can offer our thoughts and expertise. :smile:
 

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