Phase contrast microscopy Question

[gen.hope55]

I am a university student and have this question for you guys.

Why do dead cells under phase contrast microscope do not show a bright halo?

Thanks for your help
Gen.hope55

 

[Gokul43201]

Do you have any thoughts or ideas?

 

[gen.hope55]

I am asking over here because I have no clue.

 

[Andy Resnick]

I am a university student and have this question for you guys.

Why do dead cells under phase contrast microscope do not show a bright halo?

Thanks for your help
Gen.hope55

The halo comes from the fact that the phase ring (and the aperture ring) has a finite width, and is not a function of the object.

Pluta's book "Advanced Light Microscopy, vol.2- advanced methods" is the definitive text on phase contrast (and DIC as well).

This site has some good information as well:

http://www.microscopyu.com/tutorials/java/phasecontrast/shadeoff/index.html

 

[gen.hope55]

thanks Andy. Thats really helpful, I just couldn't find things at all.
Hopefully I ll be able to find more on it but you gave me what i needed.
Thanks :)
Gen.hope55