Phase contrast microscopy Question

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Dead cells do not exhibit a bright halo under a phase contrast microscope due to the nature of the phase ring and aperture ring, which have a finite width and are not influenced by the characteristics of the object being observed. For a deeper understanding of phase contrast microscopy, "Advanced Light Microscopy, vol.2 - advanced methods" by Pluta is recommended as a comprehensive resource. Additional information can be found on microscopy educational websites, which provide valuable insights into the principles of phase contrast imaging.
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I am a university student and have this question for you guys.

Why do dead cells under phase contrast microscope do not show a bright halo?

Thanks for your help
Gen.hope55
 
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Do you have any thoughts or ideas?
 
I am asking over here because I have no clue.
 
gen.hope55 said:
I am a university student and have this question for you guys.

Why do dead cells under phase contrast microscope do not show a bright halo?

Thanks for your help
Gen.hope55

The halo comes from the fact that the phase ring (and the aperture ring) has a finite width, and is not a function of the object.

Pluta's book "Advanced Light Microscopy, vol.2- advanced methods" is the definitive text on phase contrast (and DIC as well).

This site has some good information as well:

http://www.microscopyu.com/tutorials/java/phasecontrast/shadeoff/index.html
 
thanks Andy. Thats really helpful, I just couldn't find things at all.
Hopefully I ll be able to find more on it but you gave me what i needed.
Thanks :)
Gen.hope55
 
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