The choice of the sign for the Hamiltonian is of course convention, but it's a useful one, because then we have the sign defining energies and potential as it is common practice for centuries, and everybody is used to that convention. Let's take the most simple case of a non-relativistic particle moving in a potential. The Lagrangean reads
$$L=\frac{m}{2} \dot{\vec{x}}^2-V(\vec{x}).$$
The Hamiltonian thus gets (note that you should eliminate the velocities by canonical momenta ##\vec{p}=\partial_{\dot{\vec{x}}} L=m \dot{\vec{x}}##) after some simple algebra
$$H=\vec{p} \cdot \dot{\vec{x}}-L=\frac{\vec{p}^2}{2m} + V(\vec{x}).$$
That's the "right sign" in the sense that it counts kinetic energy positive and the potential thus also appears positive.
Now, indeed (as mentioned in #4) ##H## is conserved for a system that is invariant under time translations, and thanks to Noether that's the (in my opinion only!) safe ground to define, what "energy" is, namely the conserved quantity due to time-translation invariance. Noether's theorem tells us that the system is for sure time-translation invariant if the Lagrangian does not depend explicitly on time. Then indeed, using the Euler-Lagrange Equations gives
$$\dot{H}=0 \; \Rightarrow \; H=E=\text{const},$$
where ##E## is the total energy of the system.