What Does a Negative Absorbance Reading Indicate?

  • Context: High School 
  • Thread starter Thread starter rick112
  • Start date Start date
  • Tags Tags
    Absorbance Negative
Click For Summary
SUMMARY

A negative absorbance reading indicates that the spectrometer is not properly calibrated, leading to inaccurate measurements. Specifically, it suggests the presence of stimulated emission from an excited sample rather than a true absorbance value. Users should refer to the spectrometer's manual for calibration procedures, as a negative reading can mislead interpretations of sample composition. Proper calibration is essential for obtaining reliable data from spectroscopic measurements.

PREREQUISITES
  • Understanding of spectrophotometry principles
  • Familiarity with spectrometer calibration techniques
  • Knowledge of stimulated emission phenomena
  • Basic experience with absorbance and transmittance concepts
NEXT STEPS
  • Research spectrometer calibration methods and best practices
  • Learn about stimulated emission and its implications in spectroscopy
  • Explore the impact of sample preparation on absorbance readings
  • Study the specifications and manuals of specific spectrometer models
USEFUL FOR

Researchers, laboratory technicians, and students in chemistry or biochemistry who are working with spectroscopic analysis and require accurate absorbance measurements.

rick112
Messages
7
Reaction score
0
hi

i know this is kind of a basic question..but what in reality does a negative absorbance reading means?? why can't the spectrometer just say its out the reading range or something??
does a negative reading really say something can we look at it and detect something...like if the negative reading is x that means it has so much of some component...
 
Science news on Phys.org
This sounds like a question for the spectrometer's manual perhaps...?
 
that means your measurement is not well calibrated. a negative absorption from an excited sample would indicate stimulated emission
 
It seems like the spectrometer needs to be calibrated. It's like pushing the zero or tare on a balance. You manually set which point is called zero and the device reads other values with respect to that.
 

Similar threads

  • · Replies 16 ·
Replies
16
Views
6K
  • · Replies 1 ·
Replies
1
Views
2K
  • · Replies 2 ·
Replies
2
Views
2K
  • · Replies 8 ·
Replies
8
Views
10K
  • · Replies 2 ·
Replies
2
Views
1K
  • · Replies 2 ·
Replies
2
Views
2K
Replies
5
Views
2K
  • · Replies 1 ·
Replies
1
Views
1K
  • · Replies 2 ·
Replies
2
Views
2K