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jaumzaum
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I know that in prokaryotes primers are removed by a combination of RNAse-H and DNA Polimerase I, but who does that in eukaryotes?
The enzyme responsible for removing the primer in eukaryotes is called DNA polymerase delta.
DNA polymerase delta has a 3' to 5' exonuclease activity, meaning it can remove nucleotides from the 3' end of a DNA strand. This allows it to remove the RNA primer that was previously used to initiate DNA synthesis.
The primer removal by DNA polymerase delta typically occurs during the elongation phase of DNA replication, when the enzyme is actively synthesizing new DNA strands.
Yes, in addition to DNA polymerase delta, there are other enzymes involved in removing the primer in eukaryotes. These include RNase H, which specifically degrades the RNA portion of the primer, and flap endonuclease, which removes any remaining RNA or DNA fragments from the primer.
After the primer is removed, DNA polymerase delta continues to extend the DNA strand, filling in the gap left behind by the primer. The RNA fragments are then degraded by RNase H and the remaining DNA fragments are removed by flap endonuclease. The end result is a complete, newly synthesized DNA strand.