What enzyme removes the primer in EUKARYOTES

  • Thread starter jaumzaum
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In summary, the removal of the primer is necessary for DNA replication in eukaryotes. The enzyme responsible for this process is DNA polymerase I, which removes the primer using its 5' to 3' exonuclease activity and fills in the gap with DNA nucleotides. Other enzymes involved in primer removal include flap endonuclease 1 (FEN-1) and DNA ligase. The process may vary slightly between different eukaryotic cells, with some using different DNA polymerases and having variations in the functions of the enzymes involved.
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jaumzaum
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I know that in prokaryotes primers are removed by a combination of RNAse-H and DNA Polimerase I, but who does that in eukaryotes?
 
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What enzyme removes the primer in EUKARYOTES?

The enzyme responsible for removing the primer in eukaryotes is called DNA polymerase delta.

How does DNA polymerase delta remove the primer?

DNA polymerase delta has a 3' to 5' exonuclease activity, meaning it can remove nucleotides from the 3' end of a DNA strand. This allows it to remove the RNA primer that was previously used to initiate DNA synthesis.

When does the primer removal occur during DNA replication?

The primer removal by DNA polymerase delta typically occurs during the elongation phase of DNA replication, when the enzyme is actively synthesizing new DNA strands.

Are there any other enzymes involved in removing the primer?

Yes, in addition to DNA polymerase delta, there are other enzymes involved in removing the primer in eukaryotes. These include RNase H, which specifically degrades the RNA portion of the primer, and flap endonuclease, which removes any remaining RNA or DNA fragments from the primer.

What happens to the primer after it is removed?

After the primer is removed, DNA polymerase delta continues to extend the DNA strand, filling in the gap left behind by the primer. The RNA fragments are then degraded by RNase H and the remaining DNA fragments are removed by flap endonuclease. The end result is a complete, newly synthesized DNA strand.

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