- #1
nobahar
- 497
- 2
Hello!
If I run an agarose gel of undigested DNA of an organism with more than one chromosome, and obtain one band (slightly smeared), what does this mean? The chromosomes aren't all the same length (some might be approximately half the size of others). Is it because they all become "entangled?", they run a distance shorter (i.e. suggesting they are larger) than anyone chromosome. One could even speculate that the band has migrated a distance expected for the genome size, but this might be influenced by the above thought (since the resolution at that large a size makes it difficult to tell).
Any help appreciated,
Nobahar.
If I run an agarose gel of undigested DNA of an organism with more than one chromosome, and obtain one band (slightly smeared), what does this mean? The chromosomes aren't all the same length (some might be approximately half the size of others). Is it because they all become "entangled?", they run a distance shorter (i.e. suggesting they are larger) than anyone chromosome. One could even speculate that the band has migrated a distance expected for the genome size, but this might be influenced by the above thought (since the resolution at that large a size makes it difficult to tell).
Any help appreciated,
Nobahar.