- #1
mountain
- 53
- 0
Should i cover my Erlenmeyer bottle with a folie when i shake my bacteria in the shaker? Should the covering be tight or loosen?
Any suggestions?
Thanks.
Any suggestions?
Thanks.
Do you mean that i have to cover my bottle when i don't use it, under sterilizing and when i use it in shaking? How can i sterilize the inside of the bottle if i do cover it under sterilizing?Monique said:You can use cotton plugs for that purpose, when you are sterilizing your erlenmeyer you should put a cotton plug into it, so that it will become sterile too.
mountain said:Do you mean that i have to cover my bottle when i don't use it, under sterilizing and when i use it in shaking? How can i sterilize the inside of the bottle if i do cover it under sterilizing?
Thanks.
You should always cover the erlenmeyer to keep it sterile. You sterilize with heat, so it does not matter if you cover it: just don't make a tight seal so that it will explode (as moonbear mentioned).mountain said:Do you mean that i have to cover my bottle when i don't use it, under sterilizing and when i use it in shaking? How can i sterilize the inside of the bottle if i do cover it under sterilizing?
Thanks.
The purpose of covering an Erlenmeyer bottle when shaking bacteria is to prevent contamination from outside sources and to maintain a controlled environment for the bacteria to grow.
The most commonly recommended material for covering an Erlenmeyer bottle is aluminum foil. This is because it is easily accessible, inexpensive, and can be sterilized before use.
The Erlenmeyer bottle should be covered loosely to allow for air exchange, but tight enough to prevent any outside contamination. The cover should also be secured with tape or a rubber band to prevent it from falling off during shaking.
Yes, the Erlenmeyer bottle should be covered during the entire shaking process to maintain a sterile environment for the bacteria. Any interruption in the covering can lead to contamination and affect the results of the experiment.
No, it is recommended to use a new covering for each Erlenmeyer bottle to prevent cross-contamination between samples. This ensures the accuracy and reliability of the experiment results.