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mountain
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Do you have any good protocols for measuring the concentration of RNA? The ones i have they seem to be very confused.
Thanks.
Thanks.
iansmith said:If you have problem with the reading, it might be due to the fact that your RNA is not well mixed in the dilutent. You can heat up the sample at 55C for 10 minutes and then take the reading. Also make sure your DNA is well resupended in the sample you will used for measuring the concentration. Vortex the sample for about 5 sec prior to taking the reading.
RNA concentration can be measured using a variety of methods, such as spectrophotometry, fluorometry, and quantitative PCR (qPCR). These methods involve measuring the absorbance, fluorescence, or amplification of RNA molecules in a sample.
Measuring RNA concentration is important because it allows scientists to determine the amount of RNA present in a sample, which can be used to assess the quality and quantity of RNA for downstream experiments. It is also useful for comparing RNA levels between different samples or experimental conditions.
The most common units of measurement for RNA concentration are nanograms per microliter (ng/μL) or micrograms per milliliter (μg/mL). However, other units such as moles per liter (mol/L) or copies per microliter (copies/μL) may also be used.
RNA concentration can be affected by various factors, such as the type of sample, storage temperature, and exposure to RNases. It is important to follow proper sample handling and storage protocols to minimize any potential impact on RNA concentration.
While measuring RNA concentration is a useful technique, it does have limitations. For example, some methods may be more sensitive than others and may only detect a limited range of RNA concentrations. Additionally, certain contaminants or impurities in a sample may interfere with accurate measurements. It is important to choose the appropriate method and consider any potential limitations when measuring RNA concentration.