- #1
mountain
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- 0
When I make the standard curve then each standard solution is prepared like this:
800ul water
20ul each standard solution
200ul dye concentrate
The samples are prepared like this:
800ul water
4ul each sample solution
200ul dye concentrate
I wonder if I for instance get 0.5mg/ml on one of my sample. Does this mean that this value is the concentration of the original sample or should I multiply with 5 since the volume of the standard is larger (20ul) then the volume of the sample (4ul)?
800ul water
20ul each standard solution
200ul dye concentrate
The samples are prepared like this:
800ul water
4ul each sample solution
200ul dye concentrate
I wonder if I for instance get 0.5mg/ml on one of my sample. Does this mean that this value is the concentration of the original sample or should I multiply with 5 since the volume of the standard is larger (20ul) then the volume of the sample (4ul)?