- #1
Touchme
- 41
- 0
Hi,
How do I find the isoelectric point for an entire protein sequence? Such as Val-Lys-Glu-Asp-Phe.
How do I find the isoelectric point for an entire protein sequence? Such as Val-Lys-Glu-Asp-Phe.
The isoelectric point (pI) is the pH at which a molecule has a net zero charge. This is when the number of positively charged amino acids (such as lysine and arginine) equals the number of negatively charged amino acids (such as aspartic acid and glutamic acid).
The isoelectric point (pI) can be calculated using the Henderson-Hasselbalch equation, which takes into account the pKa values of the different amino acids in the molecule. The pI can also be determined experimentally by running the molecule through a pH gradient gel.
The isoelectric point (pI) is important in protein purification because it allows for the separation of proteins based on their charge. At a pH higher or lower than the pI, a protein will have a net charge and can be separated from other proteins using techniques such as ion exchange chromatography.
The amino acid sequence and structure can greatly impact the pI of a molecule. Amino acids with acidic side chains (such as aspartic acid) will decrease the pI, while basic amino acids (such as lysine) will increase the pI. Additionally, the 3-dimensional structure of the molecule can also affect the distribution of charged amino acids and therefore the pI.
Yes, the pI of a molecule can change depending on its environment. For example, changes in pH or the presence of ions can alter the distribution of charged amino acids and shift the pI. Additionally, post-translational modifications can also impact the pI of a protein.