- #1
sotellme
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to make a 25mM buffer with pH5.2 i add 25mM ethanoic acid with 25mM sodium ethanoate. after adjusting the pH, the buffer will still be 25mM.
will this also be the case if i use 25 mM Tris-base and 25 mM phosphoric acid? will i still get a buffer with 25mM after adjusting the pH to 5.2 with the phosphoric acid?
the first one is a conjugate pair (ethanoic acid and sodium ethanoate), while the second one is not a conjugate pair (Tris-base and phosphoric acid)
thanks.
will this also be the case if i use 25 mM Tris-base and 25 mM phosphoric acid? will i still get a buffer with 25mM after adjusting the pH to 5.2 with the phosphoric acid?
the first one is a conjugate pair (ethanoic acid and sodium ethanoate), while the second one is not a conjugate pair (Tris-base and phosphoric acid)
thanks.