Originally posted by Chemicalsuperfreak
GC and HPLC are great analytical tools. But can't be used for seperation. The pharmaceutical industry hates chromatography with a passion and would much rather take a hit with yield than not running a seperation.
Originally posted by nautica
I don't quite understand, why you even say that they can not be used for seperation. They are not meant for seperating, only for analyzing.
Nautica
High pressure chromatography is a technique used in analytical chemistry to separate and analyze complex mixtures of substances. It involves passing a sample through a column containing a stationary phase and a mobile phase, with the use of high pressure pumps to enhance the separation and resolution of the components.
High pressure chromatography offers several advantages over traditional chromatography techniques, including higher resolution and faster analysis times. It also allows for the separation of a wider range of compounds, including both polar and non-polar substances. Additionally, it requires smaller sample sizes and can be automated for increased efficiency.
There are several types of high pressure chromatography, including high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), and ultra-high performance liquid chromatography (UHPLC). These techniques differ in the type of stationary and mobile phases used, as well as the pressure and flow rates employed.
High pressure pumps are a critical component of high pressure chromatography, as they are responsible for creating the high pressure needed to push the sample through the column. These pumps must be able to withstand the high pressures and maintain a consistent flow rate to ensure accurate and precise separation of the components in the sample.
High pressure chromatography has a wide range of applications, including pharmaceutical analysis, food and beverage testing, environmental monitoring, and forensics. It is also commonly used in research and development to identify and purify new compounds. The versatility and high resolution of this technique make it a valuable tool in many scientific fields.