A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription. Reverse transcriptases are used by certain viruses such as HIV and hepatitis B to replicate their genomes, by retrotransposon mobile genetic elements to proliferate within the host genome, and by eukaryotic cells to extend the telomeres at the ends of their linear chromosomes. Contrary to a widely held belief, the process does not violate the flows of genetic information as described by the classical central dogma, as transfers of information from RNA to DNA are explicitly held possible.Retroviral RT has three sequential biochemical activities: RNA-dependent DNA polymerase activity, ribonuclease H (RNase H), and DNA-dependent DNA polymerase activity. Collectively, these activities enable the enzyme to convert single-stranded RNA into double-stranded cDNA. In retroviruses and retrotransposons, this cDNA can then integrate into the host genome, from which new RNA copies can be made via host-cell transcription. The same sequence of reactions is widely used in the laboratory to convert RNA to DNA for use in molecular cloning, RNA sequencing, polymerase chain reaction (PCR), or genome analysis.
I have an equation, that I used in a software I made (Delphi). Now, I want to sort of "reverse" this equation.
Y = 0-((( ClickedY * (1800 / PicHeight ))*2)-1800)
X = (( ClickedX * (1800 / PicWidth ))*2)-1800
What does ClickedY = if you know Y and the PicHeight?
what does ClickedX = if...
Someone mentioned this web page tangentially in a thread in Skepticism & Debunking, but it deserves its own thread here in GD.
http://www3.telus.net/jefmil/stairwaybackwards.htm
Everyone's heard the claim that certain songs contain hidden messages if played in reverse, but you (or at...
In a low key admission, Bush officials are allowing that they were wrong in their summary dismissal of man induced atmospheric warming.
link:www.nytimes.com/2002/06/03/science/03CLIM.html
If I understand it correctly it is possible that due to relativistic effects, 2 events A and B may be perceived by one observer as A happening before B, while for another observer B may happen before A.
Can someone give a layman explanation of under which circumstances this would happen (i.e...
The big news of the day, seems to be that tanning beds have addictive properties. Clients choose tanning beds that have some UV light to offer, because it gives them an "addictive", sense of well being.
The real news is this. Due to scares regarding skin cancers, and due to the fact that...
(Sorry, I'm testing with the 'tex' code)
Reverse Gravitational Force is the opposite force of Gravity.
It is it that keeps two bodies from not moving EXACTLY into the areas given by F=\frac {G_m_1_m_2} {r^2}...
You can test it by using EXTREMELY precise instruments, and checking if an...
Help with reverse siphon break?
Hey there,
Not sure if this is possible but...
I was wondering if there was a way to set something up so that one liquid would siphon into a lower container and stop when the liquid reached the level of the tube opening without using any sort of valve...
Ok, is there any solid, liquid, or gas that becomes heavier as it gets warmer and lighter as it gets colder? Like a reverse lava lamp.
The little physics I know says no but I Know VERY little, you know?
CJ
This is not a homework question but I am posting it here because I think it may be too basic for the other forums. I am looking for a ray diagram for a reverse galilean telescope (ie. + eyepiece, - objective, so image is minified). Can anyone describe what the ray diagram would look like or...