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dr.vj
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How do I make 7% glacial acetic acid in 40% (v/v) methanol?
dr.vj said:I am sure I will have a lot more questions to ask.
The combination of 7% glacial acetic acid and 40% methanol is often used as a solvent or reagent in experiments involving proteins, as it helps to denature and precipitate proteins for further analysis.
The concentration of a solution is typically calculated by dividing the amount of solute (in this case, glacial acetic acid) by the total volume of the solution (in this case, a mixture of 40% methanol and 60% other solvents).
Like any chemical reagent, 7% glacial acetic acid in 40% methanol should be handled with care. It is important to wear proper protective gear, such as gloves and goggles, and to work in a well-ventilated area to avoid inhaling any fumes. It is also important to follow proper storage and disposal protocols to prevent any accidents or environmental harm.
While 7% glacial acetic acid in 40% methanol is a commonly used solvent in scientific experiments, it can be substituted with other solvents depending on the specific needs of the experiment. However, it is important to take into account the potential effects on the experiment and adjust accordingly.
To prepare this solution, one would need to measure out the appropriate amounts of glacial acetic acid and methanol and mix them together. It is important to note that glacial acetic acid should always be added to the solvent, rather than the other way around, to prevent any potential reactions. The resulting solution should be thoroughly mixed before use.