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TytoAlba95
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How is PCR able to detect chromosomal translocations causing cancer?
I understand it is possible through FISH but how PCR?
I understand it is possible through FISH but how PCR?
Andrew Mason said:I think they are referring to RT-PCR. Both PCR and RT-PCR involve making copies of DNA but in RT-PCR one first uses reverse transcriptase to reverse transcribe a subject RNA strand into its DNA complement (cDNA). One then uses PCR to amplify the cDNA.
Would finding the BCR-ABL mRNA be easier than finding the fused DNA? This is not my area, but RT-PCR does seem to be used for this purpose: See for example: https://www.ncbi.nlm.nih.gov/pubmed/17705578Ygggdrasil said:No, these tests are normally done by conventional PCR, looking for chromosomal translocations at the DNA level.
Andrew Mason said:Would finding the BCR-ABL mRNA be easier than finding the fused DNA? This is not my area, but RT-PCR does seem to be used for this purpose: See for example: https://www.ncbi.nlm.nih.gov/pubmed/17705578
AM
PCR (polymerase chain reaction) is a laboratory technique used to amplify a specific DNA sequence. In cancer detection, PCR is used to identify and amplify cancer-related DNA or RNA markers, such as mutations or gene expression levels, in a small sample of tissue or body fluid.
PCR is a highly sensitive and specific technique, meaning it can accurately detect even small amounts of cancer-related DNA or RNA markers. However, the accuracy of the results depends on the quality and quantity of the sample, as well as the expertise of the lab performing the test.
PCR can be used to detect a wide range of cancers, including solid tumors and blood-related cancers. It can also be used to detect specific types of cancer, such as breast cancer or lung cancer, by targeting specific DNA or RNA markers associated with those cancers.
PCR can be used in cancer diagnosis to confirm the presence of cancer and determine its type and stage. It can also be used to monitor the effectiveness of cancer treatment by tracking changes in cancer-related DNA or RNA markers over time. Additionally, PCR can be used in research to identify potential new biomarkers for cancer detection or to develop personalized treatment plans.
While PCR is a powerful tool in cancer detection, there are some limitations and drawbacks to consider. It requires specialized equipment and trained personnel to perform, which can be costly and time-consuming. Additionally, PCR can only detect known DNA or RNA markers, so it may miss rare or unknown mutations. Finally, false positives or false negatives can occur if the sample is contaminated or if there are errors in the testing process.