Looking for the right fluorescent compound

  • Thread starter Howard Bell
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    Fluorescence
In summary, some fluorescent compounds that meet the specified criteria include Fluorescein, Rhodamine B, Coumarin 343, Tetramethylrhodamine Isothiocyanate, and Alexa Fluor 647. The Sigma-Aldrich Fluorescent Dyes & Probes catalog is also a good resource for finding these types of compounds.
  • #1
Howard Bell
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Hi I'm searching for one or more fluorescent compounds with the following characteristics:

1. High quantum efficiency > 0.85
2. 200-400nm excitation range.
3. Approximately 440nm, 625nm or 678nm emmission range
4. Decent stability (At least 30 days)

Does anyone know of a either specific compounds that meet this criteria or a good directory of fluorescent compounds?
 
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  • #2
Some fluorescent compounds that meet these criteria include:1. Fluorescein (FITC) - High quantum efficiency (0.90), 200-400nm excitation range, 520nm emission range, and good stability.2. Rhodamine B - High quantum efficiency (0.93), 200-400nm excitation range, 554nm emission range, and good stability.3. Coumarin 343 - High quantum efficiency (0.84), 200-400nm excitation range, 440nm emission range, and decent stability.4. Tetramethylrhodamine Isothiocyanate (TRITC) - High quantum efficiency (0.83), 200-400nm excitation range, 575nm emission range, and good stability.5. Alexa Fluor 647 - High quantum efficiency (0.87), 200-400nm excitation range, 678nm emission range, and good stability.A good directory of fluorescent compounds is the Sigma-Aldrich Fluorescent Dyes & Probes catalog.
 

1. What is a fluorescent compound?

A fluorescent compound is a molecule that absorbs and emits light in the visible spectrum, causing it to glow or fluoresce. These compounds are commonly used in various scientific fields for imaging and detection purposes.

2. What factors should I consider when choosing a fluorescent compound?

The most important factors to consider when choosing a fluorescent compound are its excitation and emission wavelengths, brightness, photostability, and compatibility with the sample or experimental conditions.

3. How do I determine the excitation and emission wavelengths of a fluorescent compound?

The excitation and emission wavelengths can be determined by measuring the absorbance and fluorescence spectra of the compound using a spectrophotometer or fluorometer. These instruments emit light of different wavelengths and measure the intensity of the light that is absorbed or emitted by the compound.

4. What is the difference between organic and inorganic fluorescent compounds?

Organic fluorescent compounds are composed of carbon and other elements, while inorganic fluorescent compounds are made up of elements other than carbon. Organic compounds tend to have broader excitation and emission spectra and are more commonly used in biological applications, while inorganic compounds have narrower spectra and are used in materials science and other fields.

5. Are there any safety considerations when working with fluorescent compounds?

Yes, it is important to handle fluorescent compounds with caution as some may be toxic or hazardous. It is recommended to wear appropriate personal protective equipment and follow proper disposal procedures when working with these compounds.

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