Recent content by Choisai

  1. Choisai

    Microscope magnification with camera

    I do want to calculate the total system magnification, but I want to do so using my own known or calculated values. I know the magnification of the objective I do not know the magnification of the eyepiece. Hence, I want to know the latter so I can calculate the former. So I know the following...
  2. Choisai

    Microscope magnification with camera

    Almost, the 100 mm biconvex lens here is the eyepiece. What I did was the following: I know the pixel size (3,8 micron). I push an object in its view 0,01 mm. Then I look at the distance it covered. I see that a certain point has moved 80 pixels (or something like that) That must mean it has...
  3. Choisai

    Microscope magnification with camera

    Not entirely. I used micron sized polysterene as a reference and moved these 0,01 mm. Then calculating back from that we got a magnification of 0,6 for for the eyepiece.
  4. Choisai

    Microscope magnification with camera

    It's an optical tweezer setup made by Thorlabs. Part 9 is a 100 mm biconvex lens, part 8 is a 2" long tube and 7 is the camera which has no lens. part 12 is the objective with 63X magnification. Your suggestion is what I ended up doing, and from that I was able to calculate back to the total...
  5. Choisai

    Microscope magnification with camera

    I have two lenses for my microscope. The object lens which magnifies 63 X. Then there is my eyepiece lens with f=100 mm which focuses the incoming light at an camera. How can I calculate the total magnification? All calculations so far for the eyepiece involve the 25 cm of the human eye, but now...
  6. Choisai

    Why is the the first order useful?

    It uses sound wave to change the refractive index. This index-modulation creates relatively more opaque and less opaque area, which acts like a grating. The zeroth order is made up of light coming through this grating, but I guess that isn't the part that gets refracted by the change in index...
  7. Choisai

    Why is the the first order useful?

    I get the zeroth order is not modulated, but why is the zeroth order not modulated? PS: I added an explanation of what an AOLM is to my question
  8. Choisai

    Why is the the first order useful?

    I've been looking into diffraction for AOLM and stuff like that, and these produce diffraction. Binary Spatial Light Modulators like Digital Micromirror Devices also produce diffraction. This diffraction is then focused and filtered using a pinhole. But, and here is the question: Why is always...
  9. Choisai

    Difference between polarization and phase

    So I know how polaroids and 0.5λ plates work, but does this mean that the polarization of the light and the phase of light are the same, but that the polarization of light is actually a vector description of light? Or something like that? Are these two the same?
  10. Choisai

    How Can I Normalize a Superlorentzian Function to 16-Bit?

    Thank you for your reply. The constants are indeed known. I have to find the actual values though, but for the sake of my algorithm I presume these to be known (they are actually laser beam parameters, so that is something I have yet to find out). But are these the minimum and respective maximum...
  11. Choisai

    How Can I Normalize a Superlorentzian Function to 16-Bit?

    I included the C and isolated the x. But it isn't exactly in a formula form: $$(\frac{-A}{D}-B)^(\frac{1}{n}){x_0}+C≤ x ≤ (\frac{A}{65,535-D})^(\frac{1}{n}){x_0}+C$$ How can I turn this into a formula? If I want to use it for my algorithm I need to be able to plug my x in and get a normalized...
  12. Choisai

    How Can I Normalize a Superlorentzian Function to 16-Bit?

    Hey there! I want to normalise my function. It's a superlorentzian function and looks like this: A / ( B+ [ ( (x-C)/x0 )^n ] + D A/B is maximal value of the function C is the horizontal transliteration of the function x0 refers to the width of the function (multiply this value by 8 and...
  13. Choisai

    Focused diffraction and Fourier transform

    The grating here has a single spatial frequency. The picture shows the first order, but a grating also produces the second and third order. That is why a grating also produces this cool effect: Here the light isn't monochromatic, but the grating still produces higher orders. Why aren't these...
  14. Choisai

    Focused diffraction and Fourier transform

    After searching on the web and reading a bit, I found that lenses can perform Fourier transform. All you need to do is put a transparant object in front of it, like a transparant sheet with black stripes on it and a screen behind the lens(so basically a 4f setup). The lens will then perform a...
  15. Choisai

    What are Fourier transforms of optics?

    Thank you! It explained a lot of what I wanted to know. But I was wondering: the Fourier transform of a lens looks an awful lot like diffraction of a wave that encounters a split. Is this just coincidence or is Fourier transform and diffraction two sides of the same coin? Diffraction has...
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