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How thick is the threaded 6061 aluminium plate?
Or, what length of the 316 bolt is engaged with thread cut in the 6061 aluminium?Stainless steel 316
316-7 = 700 MPa.
316-8 = 800 MPa.
Aluminium 6061.
6061-Grade = Max yield strength.
6061-O = 83 MPa.
6061-T4 =110 MPa.
6061-T6 = 240 MPa.
The 6061 aluminium has a significantly lower strength than the 316 stainless. It is in shear, so the thread will need to have a greater area, or the aluminium thread will be stripped from the hole. The weakest link will therefore be the thread cut into the 6061 aluminium. Using a nut and washer rather than threading the aluminium would lower cost of manufacture.
I would place a stainless steel thread insert in the aluminium. That increases the contact diameter and so area of thread contact, which will reduce aluminium damage due to galling or repeated disassembly. For thin sheet it could be a nut insert, nutsert, rivnut, etc. For deeper holes it could be a thread insert, helicoil or recoil, etc.
There will need to be some way of locking the assembly to prevent the bolt unscrewing.
There may need to be a lubricant used during assembly.
As a quick initial estimate of the minimum bolt size ...
316-8 has tensile strength of 800 N/mm².
Area required for 2000 N is = 2000 / 800 = 2.5 mm²;
Minimum inner radius = √(2.5/π) = 0.892 mm;
Minimum inner thread diameter = 1.78 mm.
That tells us, to handle the pull out tension we need at least a 2 mm diameter 316 bolt.
But the thread in the 6061 aluminium must have a greater diameter.
For 10 times lower yield in aluminium we need √10 times the diameter = 5.6 mm.
So at first guess, I would expect to use a minimum of a 6 mm bolt in a threaded insert.
The size of stainless steel bolt and type of threaded insert used will be determined by the length of engagement with the aluminium.