Conversion of EDX linescans to concentration

[Salish99]

I have recorded cross sections of a surface layer on a substrate in a scanning electron microscope (SEM), and took EDX (energy dispersive x-ray spectroscopy) linescans across the layer.

These are typically given in counts or cts per second.

Now, I know the average thickness of the layer from SEM and image analysis, and know the total area, so I know the volume, but I need to know how many moles or how many grams of the atoms I deposited I have to get a concentration c = g/cm^3 or c,mol = mol/cm^3. How can I convert the EDX linescans into grams or mols?

Thanks

 

[Salish99]

anyone kindly has an idea?

 

[ZapperZ]

I wish I can help, but I only use the EDX spectrum when I use the SEM only to identify the elements present, rather than to make quantitative determination of the concentration. I hope someone else who is familiar with such technique can respond.

I have a paper that may have either some technique, or at least make a reference to it, on doing such a determination. Check it out and see if it might help.

http://www.jneurosci.org/cgi/reprint/18/17/6623.pdf

Zz.

 

[Salish99]

I have a paper that may have either some technique, or at least make a reference to it, on doing such a determination. Check it out and see if it might help.
http://www.jneurosci.org/cgi/reprint/18/17/6623.pdf
Zz.

Oh, you're working on biomembranes and ion selective ionophores?
These might be of interest to you:
http://dx.doi.org/10.1016/j.bioelechem.2006.05.009
http://dx.doi.org/10.1002%2Fjcp.20476

 

[ZapperZ]

Oh, you're working on biomembranes and ion selective ionophores?
These might be of interest to you:
http://dx.doi.org/10.1016/j.bioelechem.2006.05.009
http://dx.doi.org/10.1002%2Fjcp.20476

Actually, I'm not. But they were some of the papers I read when I was about to learn the physics of SEM.

Zz.