Dichroic mirrors that reflect two wavelengths

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SUMMARY

Dichroic mirrors, specifically polychroic mirrors or dual-band dichroics, are designed to reflect two different wavelengths of light while transmitting others, enhancing fluorescence microscopy applications. Standard dichroic mirrors can be paired with notch filters to manage multiple laser light sources effectively. Additionally, dual band filter sets are available for applications requiring specific wavelength transmission. Custom manufacturing options for filters and multichroic mirrors are offered by various suppliers, catering to specialized experimental needs.

PREREQUISITES
  • Understanding of fluorescence microscopy techniques
  • Familiarity with dichroic mirrors and their applications
  • Knowledge of notch filters and their functions
  • Experience with custom optical filter manufacturing processes
NEXT STEPS
  • Research the specifications and applications of polychroic mirrors
  • Explore dual band filter sets from suppliers like Chroma and MicroscopyU
  • Investigate the design and use of custom multichroic mirrors
  • Learn about the integration of notch filters in fluorescence microscopy setups
USEFUL FOR

Fluorescence microscopy researchers, optical engineers, and laboratory technicians involved in multicolor imaging and filter design will benefit from this discussion.

gkiverm
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In fluorescence microscopy, dichroic mirrors reflect light under a critical wavelength (used to excite the sample) and transmit light over a critical wavelength (emission light from the sample). Are there mirrors that reflect two different wavelengths of light and transmit the rest? Essentially a dichroic mirror with two regions of reflection and two regions of transmittance instead of one each.
 
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Yes, these are often referred to as polychroic mirrors, dual-band dichroics, or dual- edge dichroics. If you're using two laser light sources, you could also get away with a standard dichroic to get rid of the shorter wavelength excitation light, and a notch filter to get rid of the longer wavelength excitation light.
 
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Are there also filters that only allow two ranges of wavelengths to pass through as opposed to one? Basically a band pass filter with two regions of transmission. I'm sorry, I'm having little luck finding this online for some reason.
 

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