It is effective at locating separation points because the pressure inside a separation bubble is dramatically different than in an attached boundary layer. This isn't true with a simple transition from a laminar boundary layer to a turbulent one. The pressure would change slightly owing to a change in displacement thickness (and therefore the effective shape of the airfoil), but it generally isn't very much.
Temperature may or may not be much different, but you can control that. You can, for example, heat (or cool) the model slightly so that there is still heat transfer to (from) the surface, and then when the boundary layer transitions, that heat transfer increases and it will be noticeable. With both IR and TSP, as long as the surface is not too conductive of heat, this allows a temperature gradient between the laminar and turbulent regions to exist and be visualized. In the case of a swept wing, you can even see temperature gradients that exist as a result of differential heat transfer resulting from crossflow vortices in the laminar region as well (see the paper I linked above, for example).