- #1
AmeliaSkates
- 1
- 0
I'm using a confocal optical (reflection) microscope to look at cryo (cold) specimens (held at below -20C). The objective is 1.4 cm from my sample, and separated from it by a couple of layers of glass, BUT NONETHELESS the light is melting away the ice in my samples, damaging them within 5-10 minutes! Help! I'm wondering if getting a different fiber optic light source, or installing a filter, would help.