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Need light with less heat for microscope

  1. Nov 10, 2009 #1
    I'm using a confocal optical (reflection) microscope to look at cryo (cold) specimens (held at below -20C). The objective is 1.4 cm from my sample, and separated from it by a couple of layers of glass, BUT NONETHELESS the light is melting away the ice in my samples, damaging them within 5-10 minutes! Help! I'm wondering if getting a different fiber optic light source, or installing a filter, would help.
  2. jcsd
  3. Nov 10, 2009 #2


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    Welcome to PF, Amelia.
    This might seem like a dumb question, but are you sure that the light source is to blame? Fibre optics don't generally get very warm.
  4. Nov 10, 2009 #3

    Andy Resnick

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    I agree- there are many potential sources of heat- the sample stage, for example. What is your light source?
  5. Nov 10, 2009 #4


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    If it's an incandescent source, going to an LED would help. Or, could you simply dim the source you have and still view the sample?
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