Optic microscopes. New illumination technic

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Discussion Overview

The discussion centers around a new illumination technique for optical microscopy, specifically comparing it to standard bright-field illumination. Participants explore the implications of this technique and its potential advantages, while also addressing the limitations of the information provided.

Discussion Character

  • Exploratory
  • Debate/contested
  • Technical explanation

Main Points Raised

  • One participant introduces a new illumination technique for microscopy but does not disclose specific details due to ongoing development.
  • Another participant questions the value of sharing information without details for discussion.
  • The original poster acknowledges the lack of details and expresses curiosity about the community's interest in the technique.
  • A participant raises concerns about the comparison between the two imaging methods, noting differences in the field of view and asking for the claimed advantages of the new technique.
  • The original poster suggests that the new technique primarily uses reflected light, contrasting it with bright-field illumination, which relies on refracted light.
  • One participant draws a parallel to dark field microscopy, suggesting similarities in illumination methods.
  • The original poster agrees that there are similarities to dark field microscopy but asserts that there are notable differences, particularly regarding the background illumination.

Areas of Agreement / Disagreement

Participants express varying levels of interest and skepticism regarding the new technique. While some acknowledge its potential, others highlight the lack of detailed information and clarity on its advantages compared to existing methods. The discussion remains unresolved regarding the specific benefits and characteristics of the new illumination technique.

Contextual Notes

There are limitations in the discussion due to the original poster's reluctance to share detailed information about the technique, which may affect the depth of analysis and understanding of its implications.

ramonpujals
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Dear friends

I have been working on a new way of illuminating microscope specimens. Cannot disclose the innings of it since all is under development. Nevertheless I attach photo since I think you will find it interesting. That is the mouth of a honey Bee, photo compares standard bright-field with my "special" technic. Magnification is x100. Hope you find it interesting.

Be well
 

Attachments

  • Honey Mouth Parts WM 10x10x.jpg
    Honey Mouth Parts WM 10x10x.jpg
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Science news on Phys.org
While it is indeed interesting if true, I fail to see the point of sharing on a forum if you don't give us any details to discuss.
 
You are correct. I am being egoistic since I cannot publish yet. And the purpose of the post is to know if you people find it interesting. Just out of curiosity.
I will submit full article once I end development of device.

I had qualms before posting for the very reason you mention. Yet I did it.

Yours
Ramon Pujals
 
It is unclear what the difference between the two methods is, since the field of view for the two images is apparently different. What is the claimed advantage of your technique in comparison to brightfield illumination?
 
Hi Andy

Thanks for commenting.
Claimed advantage? Well, I don't know yet. But this is what I can say:
Although the field of view is different; it is the same specimen. From Physics / light / Optics / Microscopy point of view we can see the following:

- At bright-field we have mainly refracted light. i.e. specimen refracts impinging light
- At Special we have mainly, if not only, reflected light. i.e. specimen reflects impinging light.

I think in microscopy this is paramount.

Be well
 
Dark field? Similar but not same.

Hi Drakkith

That is what the mathematic model predicted. A dark field. However there are some differences from standard dark field. First, the background should be dark, yet , it is not. Check out dark field photos.

Be well
 
Yes, that is why I said it was similar to dark field.
 

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