Q: Refractive index in phase contrast microscopy

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In phase contrast microscopy, when the refractive index of the medium matches that of the cell, visibility is limited. However, certain structures can still be observed, including membranes, vesicles, the nucleus, pigment granules, and brush borders. The discussion highlights that the refractive index of a cell is not uniform, which is crucial for the effectiveness of phase contrast techniques. This variability in refractive index allows for the visualization of cellular components despite the initial assumption that no details would be visible. The conversation also notes that while phase contrast microscopy does not enhance spatial resolution, it remains effective for identifying specific cellular structures.
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If you're using a phase contrast microscope and the refractive index of the medium is equal to the refractive index of the cell, what are you able to see? I guessed that you wouldn't see anything, but apparently that's wrong. The only options are membranes, vesicles, the nucleus, pigment granules, and a brush border.

Thank you!
 
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This sounds like homework. The question (as posed here) is nonsensical; "the" refractive index of a cell varies throughout the volume (which is why phase contrast and DIC work so well)- on the other hand, since phase contrast etc. do not increase the spatial resolution, perhaps the question can be answered that way.
 
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