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Scientists engineer E. coli that eats carbon dioxide
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[QUOTE="Ygggdrasil, post: 6268093, member: 124113"] E. coli is one of the best studied and most widely used bacteria in biotechnology. This week, published in the journal [I]Cell[/I], researchers report having engineered the bacterium to be able to generate all of its carbon from CO[sub]2[/sub], opening the way toward using these bacteria for replacement of petrochemicals in manufacturing. [URL]https://www.sciencemag.org/news/2019/11/microbe-no-longer-needs-eat-food-grow-thanks-bit-genetic-engineering[/URL] Here is a diagram summarizing the metabolism of the engineered microbe: [ATTACH type="full" alt="1575050096773.png"]253465[/ATTACH] The scientists chose to use formate as the energy source for the bacteria because formate can be prodcued electrochemically from CO2. Therefore, when combined with a carbon-free source of electricity, the bacteria could be used to generate carbon-based compounds without net carbon emissions. Previous work had generated bacteria which could incorporate a limited amount of CO2 into its biomass, but this work represents the first example where essentially all of the carbon in the bacteria comes from CO2. Generating the bacteria involved a clever combination of well thought out rational changes to the metabolism of the bacteria combined with directed evolution to introduce additional changes in the bacteria to actually get them to work as intended. Citation to published research: Gleizer et al. Conversion of Escherichia coli to Generate All Biomass Carbon from CO2. [I]Cell 179:[/I] 1255 (2019) [URL]https://www.cell.com/cell/fulltext/S0092-8674(19)31230-9[/URL] Abstract: [/QUOTE]
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Scientists engineer E. coli that eats carbon dioxide
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