Can We See Atoms and Molecules Without High Powered Microscopes?

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SUMMARY

This discussion addresses the visibility of atoms and molecules without high-powered microscopes, confirming that traditional light microscopes cannot achieve this. Atomic force microscopy (AFM) is highlighted as a method capable of imaging molecules, while optical contrast techniques can visualize materials like graphene. Additionally, advancements in fluorescence microscopy techniques, such as STED, PALM, and STORM, have significantly enhanced the ability to differentiate between atoms. The discussion also mentions the limitations of interferometric microscopes in measuring atomic layers.

PREREQUISITES
  • Understanding of atomic force microscopy (AFM)
  • Familiarity with fluorescence microscopy techniques (STED, PALM, STORM)
  • Knowledge of optical contrast principles in materials like graphene
  • Basic concepts of interferometric microscopy
NEXT STEPS
  • Research atomic force microscopy (AFM) applications in nanotechnology
  • Explore fluorescence microscopy techniques: STED, PALM, and STORM
  • Study the optical properties of graphene and its applications
  • Investigate the principles and applications of interferometric microscopy
USEFUL FOR

Researchers in nanotechnology, physicists, and anyone interested in advanced microscopy techniques for visualizing atomic and molecular structures.

viciam
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Hi guys,

I hope you don't mind me posting here, I am doing some research into atoms, molecules and microscopes. I have no knowledge of physics, the quantum world or microscopy so if my questions seem silly, please look past that and help me. Thank you

I have two questions. Is there a way to see atoms, molecules and other nanoscale objects other than through high powered microscopes?

And another question is, what are your thoughts on the FEI company microscopes? Have you used them? Would you recommend them?

Thanks in advance
 
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viciam said:
I have two questions. Is there a way to see atoms, molecules and other nanoscale objects other than through high powered microscopes?

Define "see". Are you asking for the ability to view these things with our naked eyes, without the use of any other instruments? Or are you asking if there are OTHER instruments that can be used besides "high powered microscopes"?

Zz.
 
Last edited:
You can, however, see things that are only one atomic layer thick in an optical microscope.
This becomes fairly obvious if you look at graphene, there is actually optical contrast between single and bilayer graphere, even though the difference in thickness is much less than the wavelength of the light (and you can also see the contrast between the single layer graphene and the substrate).
It is a nice -and very useful- optical effect.
(I should point out that the effect is much easier to see if you use a camera where you can adjust the contrast)
 
Interferometric microscopes can measure height contrasts down to 0.1 nm, however this can only be applied to atomic layers and not single atoms. The lateral resolution of interferometric microscopes is still the usual ~wavelength/2.

That said, enormous progress has been made in the past decade using fluorescence to differentiate between atoms. Look up the work of Stefan Hell and techniques like STED, PALM, STORM etc.

Claude.
 

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