The smallest difference in wavelength that the human eye can detect varies significantly among individuals, with some able to notice changes as small as 1-2 nm, particularly in the red/orange spectrum around 600-650 nm. Factors such as color blindness and individual sensitivity contribute to this variance, making it difficult to pinpoint a universal threshold. The brain also plays a crucial role in color perception, influencing how colors are interpreted beyond mere retinal stimulation. Intensity and proximity of colors can enhance discrimination, with a rough estimate suggesting the eye might discern changes of about 0.1% in wavelength. Overall, color perception is complex and influenced by both biological and environmental factors.