Troubleshooting DNA Gel Analysis: Standard Curve and Smallest Band Size Queries

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The discussion revolves around issues encountered in DNA gel analysis, specifically regarding the standard curve and band size representation. The user notes that their standard curve appears as a linear curve rather than a straight line, raising concerns about the accuracy of their results. Additionally, they express confusion over plotting a 0.5 band size on semi-log graph paper, where the smallest digit is 1, questioning whether to ignore this band. The user is seeking clarification on the expected linear relationship between distance from the well and the log10 value of DNA size. Overall, the discussion highlights common challenges in interpreting gel electrophoresis data and the importance of accurate graphing techniques.
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Hi everyone, I ran a gel for restriction digestion and used lamda HindIII as the marker. When I'm drawing the graph on a semi-log paper, size on y-axis and distance from the well on the x axis, the standard curve isn't a straight line, but a linear curve. Is it fine?

Another question: the smallest digit given on a semi-log graph paper for DNA size is 1, but I've got a 0.5 band and couldn't point it out on the graph. What shall I do? Shall I just ignore this?

Very much appreciated if you could help me solve my problems
 
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I'm really confused, cos I've learned that the distance from the well measured should be proportional to the log10 value of the DNA size. And by theory I should have got a straight line or nearly straight, but the curve I've got doesn't seem to be straight at all!
 
Anyone can answer my question?
 
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