What are the limits on resolution of STORM/PALM?

[Jon.G]

I know localisation microscopy 'beats' the limit on resolution by activating individual sources and rendering a final image, but what are the limits that prevent the resolution of the images of these individual fluorophores?

Is it just the number of photons collected?

Cheers

 

[Drakkith]

Try this article: https://en.wikipedia.org/wiki/Photo...scopy#Localization_of_individual_fluorophores

It looks like the limit depends on the number of photons collected, the number of background photons, the size of the CCD pixels, and more.