STORM/PALM (Stochastic Optical Reconstruction Microscopy/Photoactivated Localization Microscopy) is a super-resolution imaging technique that uses fluorescence microscopy to achieve resolutions beyond the diffraction limit of light.
STORM/PALM imaging works by using photoactivatable fluorescent probes that can be switched on and off multiple times. By imaging the same area multiple times, the location of each probe can be determined and a high-resolution image can be reconstructed.
The resolution of STORM/PALM imaging is limited by the size of the fluorescent probe used and the precision of the localization algorithm. The current limit for STORM/PALM imaging is around 10 nanometers.
STORM/PALM has the advantage of being able to image live cells and tissues without causing damage, as it uses low levels of light. It also has a higher resolution compared to other super-resolution techniques.
STORM/PALM has a wide range of potential applications, including studying the structure and dynamics of molecular interactions, imaging cellular structures and organelles, and understanding disease mechanisms at the nanoscale level.