- #1
Artermis
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Quote:
Originally Posted by Artermis
Hello Moonbear,
I don't know if you know me but I'm a relatively new member but I know that you are very knowledgeable and helpful, hopefully you'll be willing to help me with this.
Hi, I've seen you in the biology forum recently. Glad to have you on-board.
Quote:
But alas! Betanin and chloroform do not mix together, so the betanin should stay inside the cell and may not diffuse out. There should not be a visible change and the test tube should remain clear.
I don't know this specific example, so can't be entirely sure, but if you're disrupting the cells with chloroform, anything in the cell has to come out. If what comes out (betanin in your example) doesn't mix with the solvent, then it should just separate into two phases, an aqueous phase and an organic phase. The aqueous phase would contain all your water-soluble components, and the organic phase would contain all the hydrophobic compounds.
This is a perfectly good question for the bio forum, so why don't you post it there and see if anyone there has tried this, perhaps as a lab exercise in a class. They might be able to verify if what I think would happen is really what happens.
Cheers,
Moonbear
________________________________________________________
some interesting and helpful sites:
http://www.fed.cuhk.edu.hk/~johnson/tas/investigation/membrane_teacher.htm
Originally Posted by Artermis
Hello Moonbear,
I don't know if you know me but I'm a relatively new member but I know that you are very knowledgeable and helpful, hopefully you'll be willing to help me with this.
Hi, I've seen you in the biology forum recently. Glad to have you on-board.
Quote:
But alas! Betanin and chloroform do not mix together, so the betanin should stay inside the cell and may not diffuse out. There should not be a visible change and the test tube should remain clear.
I don't know this specific example, so can't be entirely sure, but if you're disrupting the cells with chloroform, anything in the cell has to come out. If what comes out (betanin in your example) doesn't mix with the solvent, then it should just separate into two phases, an aqueous phase and an organic phase. The aqueous phase would contain all your water-soluble components, and the organic phase would contain all the hydrophobic compounds.
This is a perfectly good question for the bio forum, so why don't you post it there and see if anyone there has tried this, perhaps as a lab exercise in a class. They might be able to verify if what I think would happen is really what happens.
Cheers,
Moonbear
________________________________________________________
some interesting and helpful sites:
http://www.fed.cuhk.edu.hk/~johnson/tas/investigation/membrane_teacher.htm
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