What Solvent Can Be Used to Dissolve Collagen?

[zhengw]

In acetic acid?? but I find it is difficult, which solvent I could use? thank you very much

 

[chemisttree]

hot water works fine. Water at about 100C will solubilize it. It will denature.

Undenatured collagen can be solubilized with citrate buffer at about pH of 3.4.
See here:

http://www3.interscience.wiley.com/cgi-bin/abstract/104021707/ABSTRACT?CRETRY=1&SRETRY=0

 

[zhengw]

Thank you very much.
Unfortunately, I can't open the link which you give. Is that a paper?? can you tell me the name of the title and the author.
Thank you again

 

[chemisttree]

Journal of Applied Polymer Science
Volume 13, Issue 1, Date: January 1969, Pages: 141-148
Leo D. Kahn, Lee P. Witnauer

Abstract
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer.

 

[zhengw]

I have got the paper
Thank you very much