What Solvent Can Be Used to Dissolve Collagen?

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Discussion Overview

The discussion revolves around the solvents that can be used to dissolve collagen, exploring various methods and conditions for solubilization. Participants share experiences and references related to the solubility of collagen in different solvents, including acetic acid, hot water, and citrate buffer.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested

Main Points Raised

  • One participant suggests using acetic acid but finds it difficult, prompting a search for alternative solvents.
  • Another participant claims that hot water at approximately 100°C can effectively solubilize collagen, noting that this process denatures the collagen.
  • A further claim is made that undenatured collagen can be solubilized using citrate buffer at a pH of 3.4.
  • A participant requests more information about a referenced paper, indicating difficulty accessing it and asking for details about the title and authors.
  • Details from the referenced paper are provided, including the journal name, volume, issue, and authors, which describe an automated apparatus for collagen solubilization in citrate buffer.
  • One participant confirms they have obtained the paper mentioned in the discussion.

Areas of Agreement / Disagreement

Participants present multiple approaches to dissolving collagen, with no consensus on a single best method. There are differing opinions on the effectiveness of acetic acid versus hot water and citrate buffer.

Contextual Notes

Some claims depend on specific conditions such as temperature and pH, and the effectiveness of each solvent may vary based on the type of collagen and its state (denatured vs. undenatured).

Who May Find This Useful

Researchers and practitioners in fields related to biochemistry, materials science, and biomedical engineering may find this discussion relevant, particularly those interested in collagen solubilization techniques.

zhengw
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In acetic acid?? but I find it is difficult, which solvent I could use? thank you very much
 
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hot water works fine. Water at about 100C will solubilize it. It will denature.

Undenatured collagen can be solubilized with citrate buffer at about pH of 3.4.
See here:

http://www3.interscience.wiley.com/cgi-bin/abstract/104021707/ABSTRACT?CRETRY=1&SRETRY=0
 
Thank you very much.
Unfortunately, I can't open the link which you give. Is that a paper?? can you tell me the name of the title and the author.
Thank you again
 
Journal of Applied Polymer Science
Volume 13, Issue 1, Date: January 1969, Pages: 141-148
Leo D. Kahn, Lee P. Witnauer

Abstract
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer.
 
I have got the paper
Thank you very much
 

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