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( The Force here is referring to the applied force, When moving a positive charge towards another positive charge(stationary) / field

In this formula why there is a negative sign in the formula? I am

**not asking the sign of the total workdone**at the end of the calculation, but the

**sign in the formula that is before calculation**. from where the sign comes from?does it have anything to do with the co-ordinate system,if yes then can the sign be changed if we take a different quadrant?.