Curve Crossing in Titration Experiments

[laCurva]

Hi guys,
I have been out of school for a couple of years and I forgot when is it OK to consider a curve crossing at certain value. Let me explain.
I am measuring signal in a titration experiment. The outcome is a typical saturation curve (growth curve), a hyperbola.
I am wondering if it is OK to set the curve to pass through [0,0]. I subtract the blank and so theoretically, IMO the signal at 0 should be 0.
Can you help me or direct me to a webpage where I can refresh my knowledge on this subject?
Thanks.

 

[Valerie Park]

It is generally recommended to use the origin (0,0) as the reference point when analyzing curves such as saturation curves. This is because it allows for easier comparison of the data with theoretical models. In addition, this reference point makes it easier to calculate the rate of change in the curve. You can find more information on this topic and some helpful examples here: https://www.mathworks.com/help/curvefit/references.html

 

[sir-pinski]

Hi there, great question! Curve crossing in titration experiments can be a bit tricky, but here are some general guidelines that may help you out.

Firstly, it's important to understand what a curve crossing actually means. In a titration experiment, the curve crossing occurs when the titrant (the substance being added) is in equal proportion to the analyte (the substance being measured). This is typically seen in a saturation curve, where the signal plateaus at a certain point.

Now, to address your question about setting the curve to pass through [0,0], it really depends on the specific experiment and the type of titration being performed. In some cases, it may be appropriate to set the curve to pass through [0,0], especially if the blank is subtracted as you mentioned. However, in other cases, it may be more accurate to leave the curve as is and not force it to pass through [0,0].

To determine the best approach for your specific experiment, it may be helpful to consult with a professor or a lab manual, as they may have specific guidelines for your experiment. You can also do some research online to refresh your knowledge on titration experiments and curve crossing. A good place to start may be chemistry or analytical chemistry websites, or even YouTube videos that explain the concept visually.

I hope this helps and good luck with your experiment!