Biology Lab Microscope Magnification Calculations

In summary, the student used a compound microscope with a magnification of -4530. The objective and eyepiece were attached to a 18 cm tube, with the eyepiece having a focal length of 2.62 cm. The student's near point was 24 and they could view the image with a fully relaxed eye. To find the focal length of the objective and the distance between the blood sample and the objective lens, the equations Mtotal = Mobjective*Meyepiece and L = fobjective + feyepiece were used. The equations were substituted with the given values and the problem was solved.
  • #1
rmusial91
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Homework Statement


To examine a particular blood sample in biology lab, a student uses a compound microscope set to have a magnification of -4530. The objective and eyepiece are both attached to a tube which is 18 cm in length and the eyepiece has a focal length of 2.62 cm. The near point of the person using the microscope is 24 and you can assume she can view the image produced with a fully relaxed eye.

a) What is the focal length of the objective?

b) What is the distance between the blood sample and the objective lens?


Homework Equations


Mobjective = -di/do = - di/ fobjective

Meyepiece = N/feyepiece

Mtotal = Mobjective*Meyepiece


The Attempt at a Solution



i tried using these equations...but they didnt work out for me.. unless I am using the wrong equation... i got them equation from compound microscope section of a book
 
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  • #2
The equations you have are right; you just have to do some substitution. Starting with your most general/biggest one, Mtotal = Mobjective*Meyepiece, sub in the equations that you have for the Mobjective and Meyepiece. Then, plug in the values given by the problem. You will also need to remember that L(length) = fobjective + feyepiece where the f=focal length. Hope this helps! :)
 

What is a Biology Lab Microscope?

A Biology Lab Microscope is a specialized tool used in scientific research and education to magnify and visualize small biological specimens such as cells, tissues, and microorganisms. It uses a combination of lenses and light sources to produce a magnified image of tiny structures, allowing scientists to study and analyze them in detail.

What are the main components of a Biology Lab Microscope?

The main components of a Biology Lab Microscope include an eyepiece, objective lenses, a stage, a light source, and focusing knobs. The eyepiece is where the viewer looks through to see the magnified image. The objective lenses are attached to the revolving nosepiece and provide different levels of magnification. The stage is where the specimen is placed for viewing. The light source provides illumination for the specimen, and the focusing knobs are used to adjust the focus of the image.

How do you properly use a Biology Lab Microscope?

To use a Biology Lab Microscope, start by placing the specimen on the stage and securing it with stage clips if necessary. Then, turn on the light source and adjust the intensity of the light. Next, use the lowest magnification objective lens, typically labeled as 4x, and bring it into focus using the coarse and fine focus knobs. Once the image is in focus, you can switch to higher magnification lenses for more detail. Remember to always start with the lowest magnification and work your way up. Finally, when finished, turn off the light source and return the microscope to its proper storage place.

What is the difference between a compound microscope and a stereo microscope?

A compound microscope is used for viewing thin, transparent specimens under high magnification, while a stereo microscope is used for viewing larger, solid specimens under low magnification. Compound microscopes have two sets of lenses (eyepiece and objective lenses) and use transmitted light, while stereo microscopes have two eyepieces and use reflected light. Compound microscopes are used for studying cells and tissues, while stereo microscopes are used for examining surfaces and structures of larger objects.

How do you care for and maintain a Biology Lab Microscope?

To care for and maintain a Biology Lab Microscope, always handle it with care and avoid touching the lenses. Clean the lenses with a soft, lint-free cloth or lens paper. Keep the microscope covered when not in use to protect it from dust and other particles. Regularly check and clean the light source, stage, and focusing knobs. If any part of the microscope becomes damaged or malfunctions, contact a professional for repairs. It is also important to follow the manufacturer's instructions for storage and maintenance to ensure the microscope's longevity.

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