The process catalyzed by the bacteriophage λ integrase

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In summary, the conversation discusses the steps of tranesterification that are catalyzed by the bacteriophage λ integrase. The incorrect statements (A), (B), and (D) suggest that tranesterifications involve the exchange or transfer of phosphate groups, when in fact they involve the exchange or transfer of phosphodiester bonds. Additionally, the role of bacteriophage λ integrase in catalyzing these steps is clarified.
  • #1
hidemi
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Homework Statement
Which statement is TRUE regarding the process catalyzed by the bacteriophage λ integrase?

A) Serines in the protein active site become covalently attached to the nucleic acids during the mechanism.
B) The reaction is an ATP-dependent process.
C) The reaction steps are successive tranesterifications.
D) The integrase can be classified as a hydrolase.
E) The target DNA sequence for this protein is nonspecific.

The answer is C.
Relevant Equations
NONE
Why are the steps catalyzed by the bacteriophage λ integrase successive tranesterifications?
How are (A), (B) ,(D) wrong?
 
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  • #2
A) Tranesterifications are a type of chemical reaction.B) Bacteriophage λ integrase catalyzes the steps of tranesterification.C) Tranesterifications involve exchanging the phosphate group between two molecules.D) Tranesterifications involve transferring the phosphate group between two molecules.A) Tranesterifications are not a type of chemical reaction; they are a type of enzymatic reaction.B) Bacteriophage λ integrase does not catalyze the steps of tranesterification directly; instead, it catalyzes the transfer of a phosphate group from one molecule to another molecule.C) Tranesterifications involve exchanging the phosphodiester bond between two molecules.D) Tranesterifications involve transferring the phosphodiester bond between two molecules.
 

1. What is the bacteriophage λ integrase?

The bacteriophage λ integrase is a type of enzyme found in the bacteriophage λ virus that is responsible for catalyzing the process of integration, which involves inserting the viral DNA into the host cell's genome.

2. How does the bacteriophage λ integrase work?

The bacteriophage λ integrase works by recognizing specific DNA sequences on the viral DNA and the host cell's genome, and then bringing them together to form a stable complex. This allows the viral DNA to be integrated into the host cell's genome.

3. What is the significance of the bacteriophage λ integrase?

The bacteriophage λ integrase is significant because it plays a crucial role in the life cycle of the bacteriophage λ virus. Without this enzyme, the virus would not be able to integrate its DNA into the host cell's genome and replicate.

4. How is the activity of the bacteriophage λ integrase regulated?

The activity of the bacteriophage λ integrase is regulated by a variety of factors, including the presence of specific proteins and DNA sequences that can either activate or inhibit its function. Additionally, the timing of integration is also regulated to ensure efficient viral replication.

5. What are the potential applications of studying the bacteriophage λ integrase?

Studying the bacteriophage λ integrase can provide insights into the mechanisms of DNA integration and gene regulation. This knowledge can be applied in various fields, including biotechnology and medicine, for the development of new gene editing tools and treatments for genetic diseases.

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