Recent content by query_ious

I see it on my screen... not sure how to fix this. The setup is a bit detailed but basically this is one big experiment where a mix of ~100 RNA types is pipetted into 24 boxes such that each box gets an equal amount of mix. Each box is labeled separately and then all boxes are pooled into one...

The below is motivated by a problem I'm observing in my experimental data I have m boxes, where each box is supposed to contain k molecules of mRNA. The measurement process includes labeling all the molecules with a box-specific tag, mixing them, amplifying them to detectable levels and...

mechanism #5: Statistics and/or clumping 'Passive statistics' (classic probability): Imagine a jar with two spheres, one green and one blue. Before picking any sphere, they both have equal probability to be any color. After picking the first sphere the colors of both spheres are fixed, which...

In the least invasive case, 'input' means original broadcast from the 'environmental channel', 'output' means final waveform produced by the mixer. More invasive cases allow expanding the measurements to include 'stuff in the middle', like, say, mixer input or potentiometer resistance. In...

And here was me thinking I'd figured something out lol :) Thanks Your radio example sounds suspiciously like our brain... 5 types of inputting classic sensory information (touch, taste, smell, sight, sound) which are received in distinct anatomical areas and eventually sort-of converge on...

@DennisN I like the potentionmeter example, I think it's actually a good way to represent how people think of QTL traits, especially if the underlying model is additive with no epistasis (loosely, epistasis is a nonlinear relationship between genes). Could your example be considered a limiting...

@phinds But those would all be constants like you said, right? Not variables which can in our universe take on many different values? I'm thinking more some function which takes various values depending on some set of input variables (which themselves can have different values). A toy height...

I was arguing with a friend over the whole 'big data' trend and how people are looking to get answers to questions by amassing many samples over many variables and hoping to mash it together somehow. (Under the assumption not only of lots of correlations to search over but also that...

Thanks for the replies. I'll try to clarify the experiment: 1. From one original stock, set up 8 samples with different concentrations of RNA via dilution. 2. Take a fixed volume from each sample into one well of a 192 well plate such that each well contains between 0 to 6 pg of material. This...

I'd appreciate advice on the correct statistical method to analyse a dataset - Dataset is basically a titration curve consisting of [0.5, 1, 2, 3, 4, 5, 6] pg of starting material and 8 replicates in each 'pg bin'. In 'stage 1' of the process each bin is labeled separately, in 'stage 2' all...

Thanks all! a few replies: I'm trying to figure out how to think... how to organize my mind. I'm in grad school in biology, in the 'systems' and 'immunology' departments. Over the past year (my MSc) I've built a technique to collect (dozens-hundreds of) mRNA datapoints from (1e3-1e5) single...

Thanks. Do you know if engineers intentionally design isolated systems into whatever it is they're trying to build as a control mechanism? If so, how do they go about it? Do you have any keywords I could follow up on? Quick googles lead to subjects like 'base isolation' e.g. buffering...

Hi, I've been wondering recently what types of systems/processes can give rise to independence. E.g. 'a' and 'b' are independent only given that the system constraints exist. I'm coming from biology so by independence I don't necessarily mean the strict mathematical version but something like...

Thanks for the links, I'll see what my library has and give it a go...

I know the term, though from what little I've seen it's often used as a catchphrase to replace 'we don't know why this happens'. Are you familiar with sources that give rigorous mathematical definitions, toolkits for exploring emergent behavior or, barring anything concrete, a guide on how to...